Thrombin Applications Production of fibrin clot in plasma:Typicallty one to two units of Thrombin will clot one mL of plasma.Cleavage of Fusion Proteins: Thrombin can be used for the cleavage of many peptides at the Thrombin recognition site using concentrations of 0.5 NIH units thrombin per one nanomole polypeptide in 20 microliters of 50 mM ammonium bicarbonate, pH 8.0. Thrombin cleavage of fusion proteins can be carried out at a Thrombin to fusion protein ratio of 1:500.Fusion ... Read Full Story

ScienceDaily (Nov. 9, 2009) — cystatin C Research: Declining kidney function is linked to a higher risk of heart failure, heart attack, peripheral arterial disease, and early death in individuals with or without kidney disease, according to a pair of studies appearing in an upcoming issue of the Journal of the American Society Nephrology (JASN).The findings indicate that poor kidney function may raise an individual's risk for cardiovascular complications. To evaluate heart health, clinicia... Read Full Story

Predictive value of mid-trimester amniotic fluid high-sensitive C-reactive protein, ferritin, and lactate dehydrogenase for fetal growth restriction.Borna S, Abdollahi A, Mirzaei F.Department of Gynecology and Obstetrics, Tehran University/ Medical of Science, Iran.BACKGROUND: Fetal growth restriction (FGR) is surprisingly common with placental dysfunction occurring in about 3% of pregnancies and despite advances in obstetric care, FGR remains a major problem in developed countries. AIM: The ... Read Full Story

THE RENIN SYSTEM AND ATRIAL NATRIURETIC HORMONE IN CONGESTIVE HEART FAILUREAbstract. The renin angiotensin system is activated in the majority of patients with chronic congestive heart failure of moderate to severe symptomology. Renin release may result from one of several different stimuli: renal tubular sodium delivery and sensing by the macula densa, sympathetic nervous system activity, and baroreceptor to changes in renal blood flow. Difficulties arise with an analysis of renin angioten... Read Full Story

J Anxiety Disord. 2009 Oct;23(7):973-8. Epub 2009 Jun 26This study evaluated the long-term durability of individual and group cognitive-behavioral family-based therapy (CBFT) for childhood obsessive-compulsive disorder (OCD). Thirty-eight participants (age 13-24 years) from a randomized controlled trial of individual or group CBFT for childhood OCD were assessed 7 years post-treatment. Diagnostic, symptom severity interviews and self-report measures of OCD, anxiety, and depression were admini... Read Full Story

Oocytes obtained from 85 women undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment were randomly selected for culture in media containing either human serum albumin (HSA), (n = 42) or recombinant human albumin (rHA), (n = 43). At the time of transfer, the two embryos with the overall best morphology were selected on day 2, 3 or 5. Comparable rates of fertilization, cleavage, blastocyst formation and implantation were observed in both groups. The frequency of pregnancy and early... Read Full Story

Maternal intake of omega-3 (omega-3) polyunsaturated fatty acids (PUFAs) during pregnancy has decreased, possibly contributing to a current increased risk of childhood allergy. Aim: To describe the effects of maternal omega-3 long-chain PUFA supplementation during pregnancy and lactation on the incidence of allergic disease in infancy. Methods: One hundred and forty-five pregnant women, affected by allergy themselves or having a husband or previous child with allergies, were included in a ran... Read Full Story

C-Reactive Protein Study:OBJECTIVE: Consistent epidemiologic evidence suggests that acute infections increase the risk for acute cardiovascular events. We tested in humans whether activation of peripheral leukocytes in reaction to the administration of recombinant human C-reactive protein (rhCRP) may provide a mechanism for infectious diseases to promote atherosclerotic disease. METHODS AND RESULTS: By using quantitative real-time polymerase chain reaction analysis, whole-blood expression p... Read Full Story

Wikizines are interactive magazines that anyone can create or edit - and this one is called "Human Saliva Research". Here you can find fresh voices and respond in real time. Some members write articles about recent news and trends related to the wikizine's topic, others recount relevant personal stories or share their favorite pictures and video clips. Got an interesting idea or story to share with other members of this wikizine? Well, then put on your journalist's cap and add your own arti... Read Full Story

BACKGROUND: Gamma glutamyltransferase ( GGT ) or alanine aminotransferase ( ALT) is reported to be an independent risk factor of diabetes and cardiovascular disease and proposed as a component of metabolic syndrome (MS). However, there are few studies examining the direct association between MS and Gamma glutamyltransferase or alanine aminotransferase in Japanese men and women. METHODS: Direct associations between GGT or ALT and MS defined by revised NCEP criteria for Japanese and between G... Read Full Story